Stable Cell Line Selection Marker

Stable cell line generation is made possible by the use of positive selection markers such as hygromycin g418 geneticin zeocin and blasticidin antibiotic resistance.

Stable cell line selection marker.

Selection of stable transfectants selection of stably transfected cells begins with successful transient transfection with a plasmid containing a selectable marker such as an antibiotic resistance gene. As a negative control cells should be transfected using dna that does not contain the selectable marker. The marker gene could be on either the same plasmid vector or a second co transfected vector. Selection marker in order to select stably transfected cells a selection marker must be co expressed with the target protein.

Selection markers can be delivered using the same plasmid that contains the gene of interest in cis or on a separate plasmid in trans that needs to be co transfected with the plasmid containing the gene of interest. Stable cell line generation is made possible by the use of positive selection markers such as g418 hygromycin b puromycin resistance etc. Selection markers are common genetic elements used in recombinant cell line development.